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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated EPCAM/CD326 Monoclonal antibody

EPCAM/CD326 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A2D5

Cat no : CL594-66316

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

Validation Data Gallery

Applications:
  • IF Staining of MCF-7 using CL594-66316

    IF Staining of MCF-7 using CL594-66316

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 EPCAM/CD326 antibody (CL594-66316, Clone: 2A2D5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66316-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66316 targets EPCAM/CD326 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPCAM/CD326 fusion protein Ag15393 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014785
Gene symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2883555
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Protocols

Product Specific Protocols
IF protocol for CL594 EPCAM/CD326 antibody CL594-66316Download protocol
Standard Protocols
Click here to view our Standard Protocols