CoraLite®594-conjugated EEF1D Monoclonal antibody

EEF1D Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B1B11

Cat no : CL594-60085

Synonyms

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FLJ20897, FP1047



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EEF1D Ready-To-Use IHC Kit. EEF1D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60085-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-60085 targets EEF1D in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1D fusion protein Ag0983 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated molecular weight 31 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007847
Gene symbol EEF1D
Gene ID (NCBI) 1936
RRIDAB_2883420
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Protocols

Product Specific Protocols
IF protocol for CL594 EEF1D antibody CL594-60085Download protocol
Standard Protocols
Click here to view our Standard Protocols