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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated EEF1B2 Monoclonal antibody

EEF1B2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E1C11

Cat no : CL594-60329

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-60329

    IF Staining of HeLa using CL594-60329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60329 (EEF1B2 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EEF1B2 Ready-To-Use IHC Kit. EEF1B2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60329-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60329 targets EEF1B2 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1B2 fusion protein Ag0135 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 beta 2
Calculated molecular weight 25 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC000211
Gene symbol EEF1B2
Gene ID (NCBI) 1933
RRIDAB_2883465
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL594 EEF1B2 antibody CL594-60329Download protocol
Standard Protocols
Click here to view our Standard Protocols