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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Decorin Monoclonal antibody

Decorin Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3A4E4

Cat no : 66847-1-Ig

Synonyms

Bone proteoglycan II, CSCD, DCN, decorin, DSPG2, PG S2, PG40, PGII, PGS2, SLRR1B

Validation Data Gallery

Applications:
  • WB analysis of pig cartilage using 66847-1-Ig

    WB analysis of pig cartilage using 66847-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66847-1-Ig (Decorin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 66847-1-Ig

    IF Staining of BxPC-3 using 66847-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Decorin antibody (66847-1-Ig, Clone: 3A4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig cartilage tissue
Positive IF detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66847-1-Ig targets Decorin in WB, IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Decorin fusion protein Ag6275 相同性解析による交差性が予測される生物種
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 40 kDa, 100 kDa
GenBank accession numberBC005322
Gene symbol DCN
Gene ID (NCBI) 1634
RRIDAB_2882187
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 66847-1-IgDownload protocol
IF protocol for Decorin antibody 66847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols