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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB1 Monoclonal antibody

DNAJB1 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E8E10

Cat no : 67422-1-Ig

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1

Validation Data Gallery

Applications:
  • WB analysis using 67422-1-Ig

    WB analysis using 67422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67422-1-Ig

    FC experiment of HeLa using 67422-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67422-1-Ig targets DNAJB1 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2882662
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
WB protocol for DNAJB1 antibody 67422-1-IgDownload protocol
FC protocol for DNAJB1 antibody 67422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Biol

An Important Role for RPRD1B in the Heat Shock Response

Authors - Simona Cugusi
View Article