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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

IF

Conjugate

FITC Fluorescent Dye

CloneNo.

8H6A10

Cat no : FITC-66008

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag

Validation Data Gallery

Applications:
  • IF Staining of Transfected HEK-293 using FITC-66008

    IF Staining of Transfected HEK-293 using FITC-66008

    Immunofluorescent analysis of (-20°C Ethanol) fixed with Transfected HEK-293 cells using FITC-66008 (DDDDK tag antibody) at dilution of 1:50.

Tested Applications

Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

FITC-66008 targets DYKDDDDK tag in IF applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
RRIDAB_2883827
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
IF protocol for FITC DYKDDDDK tag antibody FITC-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols