• Featured Product
  • KD/KO Validated

CoraLite®594-conjugated DBR1 Monoclonal antibody

DBR1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B5A7

Cat no : CL594-66951

Synonyms

DBR1, Lariat debranching enzyme



Tested Applications

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66951-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66951 targets DBR1 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DBR1 fusion protein Ag8830 相同性解析による交差性が予測される生物種
Full Name debranching enzyme homolog 1 (S. cerevisiae)
Calculated molecular weight 544 aa, 62 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009472
Gene symbol DBR1
Gene ID (NCBI) 51163
RRIDAB_2920062
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 DBR1 antibody CL594-66951Download protocol
Standard Protocols
Click here to view our Standard Protocols