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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cytokeratin 20 Monoclonal antibody

Cytokeratin 20 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4D10A4

Cat no : CL488-60183

Synonyms

Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT

Validation Data Gallery

Applications:
  • IF Staining of HT-29 using CL488-60183

    IF Staining of HT-29 using CL488-60183

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200.

  • IF Staining of human colon using CL488-60183

    IF Staining of human colon using CL488-60183

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200, CoraLite®594 CD68 antibody (CL594-25747, red).

Tested Applications

Positive IF detected inHT-29 cells, human colon tissue
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60183-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60183 targets Cytokeratin 20 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11336 相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
GenBank accession numberBC031559
Gene symbol KRT20
Gene ID (NCBI) 54474
RRIDAB_2919234
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-60183Download protocol
Standard Protocols
Click here to view our Standard Protocols