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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for FC (Intra), IF, WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10830

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

Validation Data Gallery

Applications:
  • WB analysis using CL594-10830

    WB analysis using CL594-10830

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-10830 (Cytokeratin 18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using CL594-10830

    IF Staining of HepG2 using CL594-10830

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Cytokeratin 18 antibody (CL594-10830) at dilution of 1:200.

  • IF Staining of HeLa using CL594-10830

    IF Staining of HeLa using CL594-10830

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Cytokeratin 18 antibody (CL594-10830) at dilution of 1:200.

  • FC experiment of HeLa using CL594-10830

    FC experiment of HeLa using CL594-10830

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Cytokeratin 18 (CL594-10830) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10830-1-AP

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-10830 targets Cytokeratin 18 in WB, IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260 相同性解析による交差性が予測される生物種
Full Name keratin 18
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000180
Gene symbol KRT18
Gene ID (NCBI) 3875
RRIDAB_2919747
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protocols

Product Specific Protocols
WB protocol for CL594 Cytokeratin 18 antibody CL594-10830Download protocol
IF protocol for CL594 Cytokeratin 18 antibody CL594-10830Download protocol
FC protocol for CL594 Cytokeratin 18 antibody CL594-10830Download protocol
Standard Protocols
Click here to view our Standard Protocols