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  • Featured Product
  • KD/KO Validated

Cyclin D3 Monoclonal antibody

Cyclin D3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F2C6

Cat no : 66357-1-Ig

Synonyms

CCND3, Cyclin D3, G1/S specific cyclin D3

Validation Data Gallery

Applications:
  • WB analysis of Raji using 66357-1-Ig

    WB analysis of Raji using 66357-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66357-1-Ig

    WB analysis of Jurkat using 66357-1-Ig

    WB result of Cyclin D3 antibody (66357-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.

  • WB analysis of HL-60 using 66357-1-Ig

    WB analysis of HL-60 using 66357-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66357-1-Ig

    WB analysis of HEK-293 using 66357-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66357-1-Ig

    WB analysis of Jurkat using 66357-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 66357-1-Ig

    WB analysis of K-562 using 66357-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 66357-1-Ig

    IHC staining of human pancreas cancer using 66357-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCND3 antibody included
  • IHC staining of human pancreas cancer using 66357-1-Ig

    IHC staining of human pancreas cancer using 66357-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCND3 antibody included

Tested Applications

Positive WB detected inRaji cells, Jurkat cells, HL-60 cells, HEK-293 cells, K-562 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CCND3 Ready-To-Use IHC Kit. CCND3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66357-1-Ig targets Cyclin D3 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cyclin D3 fusion protein Ag22825 相同性解析による交差性が予測される生物種
Full Name cyclin D3
Calculated molecular weight 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC011616
Gene symbol CCND3
Gene ID (NCBI) 896
RRIDAB_2881736
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Protocols

Product Specific Protocols
WB protocol for Cyclin D3 antibody 66357-1-IgDownload protocol
IHC protocol for Cyclin D3 antibody 66357-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Janardhan (Verified Customer) (08-22-2020)

Antibody works well for western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Medulloblastoma