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Caspase 6 Monoclonal antibody

Caspase 6 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H7F9

Cat no : 67825-1-Ig

Synonyms

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2



Tested Applications

Positive WB detected inJurkat cells
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

67825-1-Ig targets Caspase 6 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 6 fusion protein Ag20534 相同性解析による交差性が予測される生物種
Full Name caspase 6, apoptosis-related cysteine peptidase
Calculated molecular weight 33 kDa, 22 kDa
Observed molecular weight 33-35 kDa,22kDa, 18kDa
GenBank accession numberBC000305
Gene symbol CASP6
Gene ID (NCBI) 839
RRIDAB_2918587
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

Protocols

Product Specific Protocols
WB protocol for Caspase 6 antibody 67825-1-IgDownload protocol
IF protocol for Caspase 6 antibody 67825-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols