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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F8B9

Cat no : CL594-66394

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, D 28K

Validation Data Gallery

Applications:
  • IF Staining of mouse cerebellum using CL594-66394

    IF Staining of mouse cerebellum using CL594-66394

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CL594-66394 (Calbindin-D28k antibody) at dilution of 1:100.

  • IF Staining of mouse cerebellum using CL594-66394

    IF Staining of mouse cerebellum using CL594-66394

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CL594-66394 (Calbindin-D28k antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66394-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66394 targets Calbindin-D28k in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
GenBank accession numberBC006478
Gene symbol CALB1
Gene ID (NCBI) 793
RRIDAB_2883575
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
IF protocol for CL594 Calbindin-D28k antibody CL594-66394Download protocol
Standard Protocols
Click here to view our Standard Protocols