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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Calbindin-D28k Polyclonal antibody

Calbindin-D28k Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14479

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

Validation Data Gallery

Applications:
  • IF Staining of human kidney using CL488-14479

    IF Staining of human kidney using CL488-14479

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-14479) at dilution of 1:200.

  • IF Staining of human kidney using CL488-14479

    IF Staining of human kidney using CL488-14479

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-14479) at dilution of 1:200.

  • IF Staining of mouse cerebellum using CL488-14479

    IF Staining of mouse cerebellum using CL488-14479

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-14479) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman kidney tissue, mouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14479-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-14479 targets Calbindin-D28k in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
GenBank accession numberBC006478
Gene symbol CALB1
Gene ID (NCBI) 793
RRIDAB_2919092
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Calbindin-D28k antibody CL488-14479Download protocol
Standard Protocols
Click here to view our Standard Protocols