• Featured Product
  • KD/KO Validated

CYP27A1 Monoclonal antibody

CYP27A1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G3F7

Cat no : 67045-1-Ig

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase



Tested Applications

Positive WB detected inCaco-2 cells, HepG2 cells, HT-29 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

67045-1-Ig targets CYP27A1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP27A1 fusion protein Ag7746 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Calculated molecular weight 60 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC051851
Gene symbol CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2882359
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CYP27A1 antibody 67045-1-IgDownload protocol
IHC protocol for CYP27A1 antibody 67045-1-IgDownload protocol
IF protocol for CYP27A1 antibody 67045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Penthorum chinense Pursh. extract attenuates non-alcholic fatty liver disease by regulating gut microbiota and bile acid metabolism in mice.

Authors - Xiaoxi Li
mouseWB

Nat Microbiol

Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen

Authors - Jinyan Xie
humanWB

Front Immunol

Construction of a lipid metabolism-related risk model for hepatocellular carcinoma by single cell and machine learning analysis

Authors - Lisha Mou