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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

9C4D10

Cat no : CL594-66022

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66022

    IF Staining of HepG2 using CL594-66022

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66022 (CYB5R3 antibody) at dilution of 1:100.

  • IF Staining of HeLa using CL594-66022

    IF Staining of HeLa using CL594-66022

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CYB5R3 antibody (CL594-66022, Clone: 9C4D10 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66022-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66022 targets CYB5R3 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag18096 相同性解析による交差性が予測される生物種
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC004821
Gene symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_2883481
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL594 CYB5R3 antibody CL594-66022Download protocol
Standard Protocols
Click here to view our Standard Protocols