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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CX3CL1 Monoclonal antibody

CX3CL1 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5D5

Cat no : 60339-1-Ig

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 60339-1-Ig

    WB analysis of PC-3 using 60339-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60339-1-Ig (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 60339-1-Ig

    IHC staining of human brain using 60339-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 60339-1-Ig

    IHC staining of human brain using 60339-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human small intestine using 60339-1-Ig

    IHC staining of human small intestine using 60339-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human small intestine using 60339-1-Ig

    IHC staining of human small intestine using 60339-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens).

  • FC experiment of PC-3 using 60339-1-Ig

    FC experiment of PC-3 using 60339-1-Ig

    1X10^6 PC-3 cells were stained with 0.2 ug Anti-Human CX3CL1 (60339-1-Ig, Clone: 2F5D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG1 Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

60339-1-Ig targets CX3CL1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CX3CL1 fusion protein Ag0161 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) ligand 1
Calculated molecular weight 42 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001163
Gene symbol CX3CL1
Gene ID (NCBI) 6376
RRIDAB_2881448
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CX3CL1 antibody 60339-1-IgDownload protocol
IHC protocol for CX3CL1 antibody 60339-1-IgDownload protocol
FC protocol for CX3CL1 antibody 60339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

JCI Insight

Single-cell transcriptome analysis and protein profiling reveal broad immune system activation in IgG4-related disease

Authors - Chenyang Lu
View Article