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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CRP Monoclonal antibody

CRP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F10A11

Cat no : CL594-66250

Synonyms

C reactive protein, CRP, PTX1

Validation Data Gallery

Applications:
  • IF Staining of human liver using CL594-66250

    IF Staining of human liver using CL594-66250

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL594-66250 (CRP antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CRP Ready-To-Use IHC Kit. CRP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66250-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66250 targets CRP in IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CRP fusion protein Ag9883 相同性解析による交差性が予測される生物種
Full Name C-reactive protein, pentraxin-related
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC020766
Gene symbol CRP
Gene ID (NCBI) 1401
RRIDAB_2883544
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Protocols

Product Specific Protocols
IF protocol for CL594 CRP antibody CL594-66250Download protocol
Standard Protocols
Click here to view our Standard Protocols