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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CREST Monoclonal antibody

CREST Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3D5D10

Cat no : 60314-1-Ig

Synonyms

CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 60314-1-Ig

    WB analysis of NIH/3T3 using 60314-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 60314-1-Ig

    WB analysis of RAW 264.7 using 60314-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 60314-1-Ig

    WB analysis of MCF-7 using 60314-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 60314-1-Ig

    WB analysis of COLO 320 using 60314-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 60314-1-Ig

    WB analysis of HSC-T6 using 60314-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60314-1-Ig targets CREST in WB,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CREST fusion protein Ag3119 相同性解析による交差性が予測される生物種
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated molecular weight 396 aa, 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC034494
Gene symbol SS18L1
Gene ID (NCBI) 26039
RRIDAB_2881425
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).