CoraLite®594-conjugated COTL1 Monoclonal antibody
COTL1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4A3D9
Cat no : CL594-60237
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 60237-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
The immunogen of CL594-60237 is COTL1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | COTL1 fusion protein Ag1230 相同性解析による交差性が予測される生物種 |
| Full Name | coactosin-like 1 (Dictyostelium) |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC010884 |
| Gene symbol | COTL1 |
| Gene ID (NCBI) | 23406 |
| RRID | AB_2883448 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 COTL1 antibody CL594-60237 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
