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  • KD/KO Validated

CHRNA5 Monoclonal antibody

CHRNA5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G8C11

Cat no : 66363-1-Ig

Synonyms

CHRNA5, LNCR2, NACHRA5



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, C2C12 cell, pig skeletal muscle tissue, A549 cells
Positive IHC detected inhuman bladder tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66363-1-Ig targets CHRNA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHRNA5 fusion protein Ag4447 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 5
Calculated molecular weight 468 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033639
Gene symbol CHRNA5
Gene ID (NCBI) 1138
RRIDAB_2881743
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

Protocols

Product Specific Protocols
WB protocol for CHRNA5 antibody 66363-1-IgDownload protocol
IHC protocol for CHRNA5 antibody 66363-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cell Mol Life Sci

Acetylcholine promotes chronic stress-induced lung adenocarcinoma progression via α5-nAChR/FHIT pathway

Authors - Yang Jiao
  • KD Validated
human,mouseIHC

J Invest Dermatol

Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.

Authors - Jing Wang
  • KD Validated
humanIHC

Carcinogenesis

α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.

Authors - Qian Zhang
humanIHC

Mol Carcinog

PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma

Authors - Qiang Li
humanIHC

Toxicology

The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration.

Authors - Ying Jia
humanIHC

Hum Cell

The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion.

Authors - Ping Zhu