- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated CDK4 Polyclonal antibody
CDK4 Polyclonal Antibody for FC, IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11026
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11026-1-AP
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-11026 targets CDK4 in IF, FC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDK4 fusion protein Ag1505 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase 4 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC010153 |
| Gene symbol | CDK4 |
| Gene ID (NCBI) | 1019 |
| RRID | AB_2919013 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CDK4 antibody CL488-11026 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
