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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD68 Polyclonal antibody

CD68 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-25747

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Validation Data Gallery

Applications:
  • IF Staining of human colon using CL594-25747

    IF Staining of human colon using CL594-25747

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200, Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), Collagen Type III (N-terminal) antibody (22734-1-AP, green).

  • IF Staining of human tonsillitis using CL594-25747

    IF Staining of human tonsillitis using CL594-25747

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-25747

    IF Staining of human tonsillitis using CL594-25747

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

  • IF Staining of human colon using CL594-25747

    IF Staining of human colon using CL594-25747

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

  • IF Staining of human colon using CL594-25747

    IF Staining of human colon using CL594-25747

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

  • FC experiment of RAW 264.7 using CL594-25747

    FC experiment of RAW 264.7 using CL594-25747

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD68 (CL594-25747) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman colon tissue, human tonsillitis tissue
Positive FC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25747-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of CL594-25747 is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
RRIDAB_2919875
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Protocols

Product Specific Protocols
IF protocol for CL594 CD68 antibody CL594-25747Download protocol
FC protocol for CL594 CD68 antibody CL594-25747Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Mol Biosci

Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.

Authors - Peng Zou
View Article