CD4 Monoclonal antibody

CD4 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (4)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4A2

Cat no : 67786-1-Ig

Synonyms

CD4, CD4 molecule, CD4mut



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:450-1:1800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67786-1-Ig targets CD4 in IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD4 fusion protein Ag30870 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
RRIDAB_2918550
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IHC protocol for CD4 antibody 67786-1-IgDownload protocol
IF protocol for CD4 antibody 67786-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

J Med Virol

Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy

Authors - Xinyue Deng
mouseIF

Cardiovasc Res

Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism

Authors - Yang Lu
humanIHC

Clin Transl Med

Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy

Authors - Qing Wang
human,mouseIHC

Front Immunol

LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma

Authors - Houhong Wang
humanIHC

Front Immunol

Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma

Authors - Qinyang Wang
mouseIF

Immunology

Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis

Authors - Jingjing Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate