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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4A7G3

Cat no : CL647-60271

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL647-60271

    IF Staining of human tonsillitis using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL647-60271 (CD20 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human lung cancer using CL647-60271

    IF Staining of human lung cancer using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

  • IF Staining of human tonsillitis using CL647-60271

    IF Staining of human tonsillitis using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL647-60271 (CD20 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human lung cancer using CL647-60271

    IF Staining of human lung cancer using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

  • IF Staining of human lung cancer using CL647-60271

    IF Staining of human lung cancer using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 pan-keratin antibody (CL488-26411, green).

  • FC experiment of Raji using CL647-60271

    FC experiment of Raji using CL647-60271

    1X10^6 Raji cells were stained with 0.20 ug CoraLite® Plus 647-conjugated Anti-Human CD20 (CL647-60271, Clone:4A7G3) (red) or 0.20 ug control antibody (blue). Fixed with90% MeOH.

Tested Applications

Positive IF detected inhuman tonsillitis tissue, human lung cancer tissue
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60271-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

CL647-60271 targets CD20 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
GenBank accession numberBC002807
Gene symbol MS4A1
Gene ID (NCBI) 931
RRIDAB_2883651
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD20 antibody CL647-60271Download protocol
FC protocol for CL Plus 647 CD20 antibody CL647-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols