CD133 Monoclonal antibody
CD133 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2B8A2
Cat no : 66666-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, Caco-2 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 28 publications below |
| IHC | See 10 publications below |
| IF | See 15 publications below |
| ELISA | See 1 publications below |
Product Information
66666-1-Ig targets CD133 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD133 fusion protein Ag13327 相同性解析による交差性が予測される生物種 |
| Full Name | prominin 1 |
| Calculated molecular weight | 97 kDa |
| Observed molecular weight | 115 kDa, 80-90 kDa |
| GenBank accession number | BC012089 |
| Gene symbol | PROM1 |
| Gene ID (NCBI) | 8842 |
| RRID | AB_2801586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD133 antibody 66666-1-Ig | Download protocol |
| IHC protocol for CD133 antibody 66666-1-Ig | Download protocol |
| FC protocol for CD133 antibody 66666-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma | ||
Mol Ther Oncolytics ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer. | ||
Pharmacol Res Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B. | ||
Int J Mol Sci Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma | ||
Int J Mol Sci Cell-Surface GRP78-Targeted Chimeric Antigen Receptor T Cells Eliminate Lung Cancer Tumor Xenografts |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (08-16-2023) | Worked well in WB at 1:1000
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