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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD11c/Integrin Alpha X Monoclonal antibody

CD11c/Integrin Alpha X Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F1C10

Cat no : CL594-60258

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL594-60258

    IF Staining of human tonsillitis using CL594-60258

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-60258

    IF Staining of human tonsillitis using CL594-60258

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-60258

    IF Staining of human tonsillitis using CL594-60258

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10 ) at dilution of 1:200, CoraLite®488 CD8 antibody (CL488-66868, Clone: 1G2B10, green).

Tested Applications

Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60258-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-60258 is CD11c/Integrin Alpha X Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD11c/Integrin Alpha X fusion protein Ag11350 相同性解析による交差性が予測される生物種
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
GenBank accession numberBC038237
Gene symbol ITGAX
Gene ID (NCBI) 3687
RRIDAB_2883450
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL594 CD11c/Integrin Alpha X antibody CL594-60258Download protocol
Standard Protocols
Click here to view our Standard Protocols