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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD11a/Integrin Alpha L Monoclonal antibody

CD11a/Integrin Alpha L Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E3H12

Cat no : 66256-1-Ig

Synonyms

CD11A, CD11a/Integrin Alpha L, CD11a/Integrin alpha-L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

Validation Data Gallery

Applications:
  • WB analysis of Ramos using 66256-1-Ig

    WB analysis of Ramos using 66256-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66256-1-Ig

    IHC staining of human tonsillitis using 66256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66256-1-Ig

    IHC staining of human tonsillitis using 66256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 66256-1-Ig

    IF Staining of human tonsillitis using 66256-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 66256-1-Ig

    IF Staining of human tonsillitis using 66256-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inRamos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66256-1-Ig targets CD11a/Integrin Alpha L in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD11a/Integrin Alpha L fusion protein Ag7813 相同性解析による交差性が予測される生物種
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated molecular weight 129 kDa
Observed molecular weight180 kDa
GenBank accession numberBC008777
Gene symbol ITGAL
Gene ID (NCBI) 3683
RRIDAB_2881644
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
WB protocol for CD11a/Integrin Alpha L antibody 66256-1-IgDownload protocol
IHC protocol for CD11a/Integrin Alpha L antibody 66256-1-IgDownload protocol
IF protocol for CD11a/Integrin Alpha L antibody 66256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols