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- KD/KO Validated
Caveolin-1 Monoclonal antibody
Caveolin-1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
6C2B2
Cat no : 66067-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, A431 cells, PC-3 cells, human heart tissue, pig heart tissue, Rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver cancer tissue, human skin cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Product Information
66067-1-Ig targets Caveolin-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, sheep, mouse, canine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Caveolin-1 fusion protein Ag8049 相同性解析による交差性が予測される生物種 |
| Full Name | caveolin 1, caveolae protein, 22kDa |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 20-25 kDa |
| GenBank accession number | BC006432 |
| Gene symbol | CAV1 |
| Gene ID (NCBI) | 857 |
| RRID | AB_11043343 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caveolin-1 antibody 66067-1-Ig | Download protocol |
| IHC protocol for Caveolin-1 antibody 66067-1-Ig | Download protocol |
| IF protocol for Caveolin-1 antibody 66067-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation | ||
Mol Metab CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue. | ||
Cells Blocking EREG/GPX4 Sensitizes Head and Neck Cancer to Cetuximab through Ferroptosis Induction | ||
Food Chem Toxicol α-Hemolysin suppresses osteogenesis by inducing lipid rafts accumulation in bone marrow stromal cells. | ||
Proteomics Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes. |
