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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARS Monoclonal antibody

CARS Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F7E4

Cat no : 67933-1-Ig

Synonyms

CARS, CARS1, CYSRS, Cysteine tRNA ligase, cysteinyl tRNA synthetase

Validation Data Gallery

Applications:
  • WB analysis using 67933-1-Ig

    WB analysis using 67933-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 67933-1-Ig

    WB analysis using 67933-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of A549 using 67933-1-Ig

    WB analysis of A549 using 67933-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67933-1-Ig

    IF Staining of HepG2 using 67933-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CARS antibody (67933-1-Ig, Clone: 2F7E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, A549 cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67933-1-Ig targets CARS in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CARS fusion protein Ag7651 相同性解析による交差性が予測される生物種
Full Name cysteinyl-tRNA synthetase
Calculated molecular weight 89 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC002880
Gene symbol CARS
Gene ID (NCBI) 833
RRIDAB_2918685
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CARS antibody 67933-1-IgDownload protocol
IF protocol for CARS antibody 67933-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols