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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAPNS1 Monoclonal antibody

CAPNS1 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2B6G6

Cat no : 67838-1-Ig

Synonyms

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

Validation Data Gallery

Applications:
  • WB analysis of A375 using 67838-1-Ig

    WB analysis of A375 using 67838-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67838-1-Ig

    WB analysis of HeLa using 67838-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 67838-1-Ig

    WB analysis of K-562 using 67838-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 67838-1-Ig

    WB analysis of THP-1 using 67838-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67838-1-Ig

    WB analysis of HSC-T6 using 67838-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 67838-1-Ig

    WB analysis of PC-12 using 67838-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 67838-1-Ig

    WB analysis of A431 using 67838-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67838-1-Ig

    IF Staining of A431 using 67838-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPNS1 antibody (67838-1-Ig, Clone: 2B6G6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, A431 cells, HeLa cells, K-562 cells, THP-1 cells, HSC-T6 cells, PC-12 cells
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CAPNS1 Ready-To-Use IHC Kit. CAPNS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67838-1-Ig targets CAPNS1 in WB, IF,ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPNS1 fusion protein Ag21885 相同性解析による交差性が予測される生物種
Full Name calpain, small subunit 1
Calculated molecular weight 28 kDa
GenBank accession numberBC011903
Gene symbol CAPNS1
Gene ID (NCBI) 826
RRIDAB_2918600
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAPNS1 antibody 67838-1-IgDownload protocol
IF protocol for CAPNS1 antibody 67838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols