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  • Featured Product
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BRD8 Monoclonal antibody

BRD8 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5B6D6

Cat no : 60121-1-Ig

Synonyms

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 60121-1-Ig

    WB analysis of HeLa using 60121-1-Ig

    WB result of BRD8 antibody (60121-1-Ig, 1:8000) with si-Control and si-BRD8 transfected HeLa cells..

  • WB analysis of fetal human brain using 60121-1-Ig

    WB analysis of fetal human brain using 60121-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-Ig (BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 60121-1-Ig

    IHC staining of human colon cancer using 60121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 60121-1-Ig

    IHC staining of human colon cancer using 60121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 60121-1-Ig

    IF Staining of HEK-293 using 60121-1-Ig

    Immunofluorescent analysis of (4% PFA ) fixed HEK-293 cells using 60121-1-Ig(BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 60121-1-Ig

    IF Staining of HepG2 using 60121-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD8 antibody (60121-1-Ig, Clone: 5B6D6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

60121-1-Ig targets BRD8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BRD8 fusion protein Ag0790 相同性解析による交差性が予測される生物種
Full Name bromodomain containing 8
Calculated molecular weight 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC008039
Gene symbol BRD8
Gene ID (NCBI) 10902
RRIDAB_2066025
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].

Protocols

Product Specific Protocols
WB protocol for BRD8 antibody 60121-1-IgDownload protocol
IHC protocol for BRD8 antibody 60121-1-IgDownload protocol
IF protocol for BRD8 antibody 60121-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols