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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated BORIS Monoclonal antibody

BORIS Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B12E2

Cat no : CL594-60079

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-60079

    IF Staining of HeLa using CL594-60079

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 BORIS antibody (CL594-60079, Clone: 2B12E2 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CTCFL/BORIS Ready-To-Use IHC Kit. CTCFL/BORIS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60079-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60079 targets BORIS in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BORIS fusion protein Ag1003 相同性解析による交差性が予測される生物種
Full Name CCCTC-binding factor (zinc finger protein)-like
Calculated molecular weight 82 kDa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC130486
Gene symbol CTCFL
Gene ID (NCBI) 140690
RRIDAB_2883418
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Protocols

Product Specific Protocols
IF protocol for CL594 BORIS antibody CL594-60079Download protocol
Standard Protocols
Click here to view our Standard Protocols