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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BLNK Monoclonal antibody

BLNK Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A1

Cat no : 66953-1-Ig

Synonyms

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

Validation Data Gallery

Applications:
  • WB analysis of human spleen using 66953-1-Ig

    WB analysis of human spleen using 66953-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Ramos using 66953-1-Ig

    WB analysis of Ramos using 66953-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig spleen using 66953-1-Ig

    WB analysis of pig spleen using 66953-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 66953-1-Ig

    WB analysis of Raji using 66953-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Daudi using 66953-1-Ig

    WB analysis of Daudi using 66953-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 66953-1-Ig

    IHC staining of human appendicitis using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IHC staining of human appendicitis using 66953-1-Ig

    IHC staining of human appendicitis using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IHC staining of mouse thymus using 66953-1-Ig

    IHC staining of mouse thymus using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IHC staining of mouse thymus using 66953-1-Ig

    IHC staining of mouse thymus using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IHC staining of rat spleen using 66953-1-Ig

    IHC staining of rat spleen using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IHC staining of rat spleen using 66953-1-Ig

    IHC staining of rat spleen using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IF Staining of Raji using 66953-1-Ig

    IF Staining of Raji using 66953-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman spleen tissue, Raji cells, Daudi cells, Ramos cells, pig spleen tissue
Positive IHC detected inhuman appendicitis tissue, mouse thymus tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy BLNK Ready-To-Use IHC Kit. BLNK primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66953-1-Ig targets BLNK in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BLNK fusion protein Ag28673 相同性解析による交差性が予測される生物種
Full Name B-cell linker
Calculated molecular weight 50 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC018906
Gene symbol BLNK
Gene ID (NCBI) 29760
RRIDAB_2882276
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

Protocols

Product Specific Protocols
WB protocol for BLNK antibody 66953-1-IgDownload protocol
IHC protocol for BLNK antibody 66953-1-IgDownload protocol
IF protocol for BLNK antibody 66953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols