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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H8C6

Cat no : CL594-60178

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-60178

    IF Staining of HeLa using CL594-60178

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60178 (BCL2 antibody) at dilution of 1:100.

  • FC experiment of Jurkat using CL594-60178

    FC experiment of Jurkat using CL594-60178

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BCL2 (CL594-60178, Clone:4H8C6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60178-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-60178 targets BCL2 in IF, FC (Intra) applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2883434
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Protocols

Product Specific Protocols
IF protocol for CL594 BCL2 antibody CL594-60178Download protocol
FC protocol for CL594 BCL2 antibody CL594-60178Download protocol
Standard Protocols
Click here to view our Standard Protocols