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- KD/KO Validated
BAX Monoclonal antibody
BAX Monoclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ICC, FC, CoIP, ELISA
Conjugate
Unconjugated
352
CloneNo.
4G5E8
Cat no : 60267-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human testis tissue, HepG2 cells, HEK-293 cells, COLO 320 cells, PC-12 cells, ROS1728 cells, Neuro-2a cells |
Positive IP detected in | THP-1 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 335 publications below |
IHC | See 20 publications below |
IF | See 17 publications below |
CoIP | See 1 publications below |
Product Information
60267-1-Ig targets BAX in WB, IP, IHC, IF, ICC, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse, pig, canine |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | BAX fusion protein Ag21068 相同性解析による交差性が予測される生物種 |
Full Name | BCL2-associated X protein |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC014175 |
Gene symbol | BAX |
Gene ID (NCBI) | 581 |
RRID | AB_2848213 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for BAX antibody 60267-1-Ig | Download protocol |
IHC protocol for BAX antibody 60267-1-Ig | Download protocol |
IP protocol for BAX antibody 60267-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
J Adv Res Evolocumab prevents atrial fibrillation in rheumatoid arthritis rats through restraint of PCSK9 induced atrial remodeling | ||
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. | ||
Cell Death Dis DUSP1 protects against ischemic acute kidney injury through stabilizing mtDNA via interaction with JNK | ||
Theranostics Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. | ||
Cell Prolif OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy |