• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ART1 Monoclonal antibody

ART1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D10A8

Cat no : 66958-1-Ig

Synonyms

ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6

Validation Data Gallery

Applications:
  • WB analysis using 66958-1-Ig

    WB analysis using 66958-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66958-1-Ig (ART1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inEC109 cells, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66958-1-Ig targets ART1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ART1 fusion protein Ag28269 相同性解析による交差性が予測される生物種
Full Name ADP-ribosyltransferase 1
Calculated molecular weight 36 kDa
Observed molecular weight 34-36 kDa
GenBank accession numberNM_004314
Gene symbol ART1
Gene ID (NCBI) 417
RRIDAB_2882281
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.

Protocols

Product Specific Protocols
WB protocol for ART1 antibody 66958-1-IgDownload protocol
IHC protocol for ART1 antibody 66958-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Med Oncol

Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis.

Authors - Shuxian Zhang
View Article