AQP1 Monoclonal antibody

AQP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E5D6

Cat no : 66805-1-Ig

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel



Tested Applications

Positive WB detected inhuman heart tissue, human skeletal muscle tissue, pig kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy AQP1 Ready-To-Use IHC Kit. AQP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

66805-1-Ig targets AQP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AQP1 fusion protein Ag14093 相同性解析による交差性が予測される生物種
Full Name aquaporin 1 (Colton blood group)
Calculated molecular weight 269 aa, 29 kDa
Observed molecular weight 28 kDa, 38-40 kDa
GenBank accession numberBC022486
Gene symbol AQP1
Gene ID (NCBI) 358
RRIDAB_2882148
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

Protocols

Product Specific Protocols
WB protocol for AQP1 antibody 66805-1-IgDownload protocol
IHC protocol for AQP1 antibody 66805-1-IgDownload protocol
IF protocol for AQP1 antibody 66805-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
humanIF

Adv Sci (Weinh)

Behind the Indolent Facade: Uncovering the Molecular Features and Malignancy Potential in Lung Minimally Invasive Adenocarcinoma by Single-Cell Transcriptomics

Authors - Xin Zhang