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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated APOL1-Specific Monoclonal antibody

APOL1-Specific Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G12D11

Cat no : CL594-66124

Synonyms

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66124

    IF Staining of HepG2 using CL594-66124

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 APOL1-Specific antibody (CL594-66124, Clone: 1G12D11 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66124-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66124 is APOL1-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APOL1-Specific fusion protein Ag2016 相同性解析による交差性が予測される生物種
Full Name apolipoprotein L, 1
Calculated molecular weight 44 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC017331
Gene symbol APOL1
Gene ID (NCBI) 8542
RRIDAB_2883518
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

Protocols

Product Specific Protocols
IF protocol for CL594 APOL1-Specific antibody CL594-66124Download protocol
Standard Protocols
Click here to view our Standard Protocols