Apolipoprotein A II Polyclonal antibody

Apolipoprotein A II Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16845-1-AP

Synonyms

Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 16845-1-AP is Apolipoprotein A II Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein A II fusion protein Ag9863 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-II
Calculated molecular weight 100 aa, 11 kDa
Observed molecular weight 7-9 kDa (monomer), 15 kDa (dimer)
GenBank accession numberBC005282
Gene symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2878324
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IHC protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IF protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IP protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Authors - Yanying Wang
humanWB

Exp Cell Res

Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.

Authors - Jun-Jun Qiu
human,mouseWB

Int J Mol Sci

Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes

Authors - Zhonghao Li