CoraLite®594-conjugated APBB1 Monoclonal antibody

APBB1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B9G10

Cat no : CL594-67077

Synonyms

APBB1, FE65, Protein Fe65, RIR



Tested Applications

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 67077-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67077 targets APBB1 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APBB1 fusion protein Ag27436 相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Calculated molecular weight 708 aa, 77 kDa
GenBank accession numberBC010854
Gene symbol APBB1
Gene ID (NCBI) 322
RRIDAB_2920084
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).

Protocols

Product Specific Protocols
IF protocol for CL594 APBB1 antibody CL594-67077Download protocol
Standard Protocols
Click here to view our Standard Protocols