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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APBB1 Monoclonal antibody

APBB1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B9G10

Cat no : 67077-1-Ig

Synonyms

APBB1, FE65, Protein Fe65, RIR

Validation Data Gallery

Applications:
  • WB analysis using 67077-1-Ig

    WB analysis using 67077-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67077-1-Ig (APBB1 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67077-1-Ig

    IHC staining of mouse cerebellum using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 67077-1-Ig

    IHC staining of mouse cerebellum using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67077-1-Ig

    IHC staining of mouse brain using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67077-1-Ig

    IHC staining of mouse brain using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 67077-1-Ig

    IF Staining of mouse brain using 67077-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using APBB1 antibody (67077-1-Ig, Clone: 1B9G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, Pig brain, Rat brain, Mouse brain
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67077-1-Ig targets APBB1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APBB1 fusion protein Ag27436 相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Calculated molecular weight 708 aa, 77 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC010854
Gene symbol APBB1
Gene ID (NCBI) 322
RRIDAB_2882385
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).

Protocols

Product Specific Protocols
WB protocol for APBB1 antibody 67077-1-IgDownload protocol
IHC protocol for APBB1 antibody 67077-1-IgDownload protocol
IF protocol for APBB1 antibody 67077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols