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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP5Z1/SPG48 Monoclonal antibody

AP5Z1/SPG48 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G1D7

Cat no : 66533-1-Ig

Synonyms

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

Validation Data Gallery

Applications:
  • WB analysis using 66533-1-Ig

    WB analysis using 66533-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66533-1-Ig

    IHC staining of human liver cancer using 66533-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66533-1-Ig

    IHC staining of human liver cancer using 66533-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 66533-1-Ig

    IF Staining of HEK-293 using 66533-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, Caco-2 cells, COLO 320 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66533-1-Ig targets AP5Z1/SPG48 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AP5Z1/SPG48 fusion protein Ag26961 相同性解析による交差性が予測される生物種
Full Name KIAA0415
Calculated molecular weight 807 aa, 88 kDa
Observed molecular weight 88 kDa, 160 kDa
GenBank accession numberBC037399
Gene symbol KIAA0415
Gene ID (NCBI) 9907
RRIDAB_2881896
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.

Protocols

Product Specific Protocols
WB protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
IHC protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
IF protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols