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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPD2 Monoclonal antibody

AMPD2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

2C4C1

Cat no : 67430-1-Ig

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2

Validation Data Gallery

Applications:
  • WB analysis using 67430-1-Ig

    WB analysis using 67430-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67430-1-Ig

    WB analysis using 67430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67430-1-Ig

    WB analysis of HepG2 using 67430-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 67430-1-Ig

    WB analysis of L02 using 67430-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67430-1-Ig

    WB analysis of HSC-T6 using 67430-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 67430-1-Ig

    WB analysis of 4T1 using 67430-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67430-1-Ig

    IHC staining of human liver cancer using 67430-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67430-1-Ig

    IHC staining of human liver cancer using 67430-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67430-1-Ig

    IF Staining of HepG2 using 67430-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPD2 antibody (67430-1-Ig, Clone: 2C4C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHSC-T6 cells, 4T1 cells, HeLa cells, HepG2 cells, L02 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67430-1-Ig targets AMPD2 in WB, IHC, IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AMPD2 fusion protein Ag8433 相同性解析による交差性が予測される生物種
Full Name adenosine monophosphate deaminase 2 (isoform L)
Calculated molecular weight879aa,101 kDa; 798aa,92 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC007711
Gene symbol AMPD2
Gene ID (NCBI) 271
RRIDAB_2882668
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

Protocols

Product Specific Protocols
WB protocol for AMPD2 antibody 67430-1-IgDownload protocol
IHC protocol for AMPD2 antibody 67430-1-IgDownload protocol
IF protocol for AMPD2 antibody 67430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols