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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ALDOA Monoclonal antibody

ALDOA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D6C2

Cat no : CL488-67453

Synonyms

ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-67453

    IF Staining of HeLa using CL488-67453

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ALDOA antibody (CL488-67453, Clone: 1D6C2 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67453-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67453 targets ALDOA in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDOA fusion protein Ag17952 相同性解析による交差性が予測される生物種
Full Name aldolase A, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC016800
Gene symbol ALDOA
Gene ID (NCBI) 226
RRIDAB_2919478
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ALDOA antibody CL488-67453Download protocol
Standard Protocols
Click here to view our Standard Protocols