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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-29 protein

Activity

0.12-0.6 ng/mL EC50

Specific Activity

>8.00 x 105 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1156

Synonyms

Cytokine Zcyto21, IFN lambda 1, IFNL1, IFN-lambda 1, IL 29, IL29, IL-29, Interferon lambda 1, Interleukin 29, ZCYTO21

Validation Data Gallery

  • Purity of recombinant human IL-29 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-29 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-29 (HZ-1156) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range is 0.12-0.6 ng/mL.

    Recombinant human IL-29 (HZ-1156) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range is 0.12-0.6 ng/mL.

Technical Specifications

GeneID 282618
Species Human
Expression HEK293
Activity 0.12-0.6 ng/mL EC50
Specific Activity>8.00 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ8IU54
Molecular Mass 27 and 31 kDa reduced, 25 and 30 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-29（IL-29, also known as IFN gamma1）is a member of the helical cytokine family and is a type III interferon. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses (PMID: 18547367;12469119;27637354)

Synonyms

Cytokine Zcyto21, IFN lambda 1, IFNL1, IFN-lambda 1, IL 29, IL29, IL-29, Interferon lambda 1, Interleukin 29, ZCYTO21

Publications

SpeciesTitle

Int J Mol Sci

Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes.

Authors - Kota Tachibana
View Article

Antiviral Res

Antiviral candidates against the hepatitis E virus (HEV) and their combinations inhibit HEV growth in in vitro.

Authors - Takashi Nishiyama
View Article
human

Cell Rep

Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.

Authors - Shasha Liu
View Article
human

J Virol Methods

Screening of novel drugs for inhibiting hepatitis E virus replication.

Authors - Takashi Nishiyama
View Article