• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-4 protein

Activity

≤ 1.25 ng/mL EC50 in Balb/c 3T3 cells. 6-30 ng/mL EC50 in NIH 3T3 cells in defined media

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1218

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3



Technical Specifications

GeneID 2249
Species Human
Expression HEK293
Activity ≤ 1.25 ng/mL EC50 in Balb/c 3T3 cells. 6-30 ng/mL EC50 in NIH 3T3 cells in defined media
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP11403
Molecular Mass 17 and 27 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4.

Background

Fibroblast growth factor-4 (FGF-4) plays an important physiologically role in the self-renewal of human embryonic stem cells (PMID 21990129). It also promotes stem cell proliferation and may aid in differentiation. Xeno-free Recombinant Human FGF-4 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 17 and 27 kDa.

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

Publications

SpeciesTitle
mouse

J Cell Commun Signal

Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.

Authors - Yin Cao