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  • Featured Product
  • KD/KO Validated

SMN Polyclonal antibody

SMN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20451-1-AP

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 20451-1-AP

    WB analysis of HeLa using 20451-1-AP

    WB result of SMN antibody (20451-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

  • WB analysis of HEK-293 using 20451-1-AP

    WB analysis of HEK-293 using 20451-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 20451-1-AP

    WB analysis of Jurkat using 20451-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20451-1-AP

    WB analysis of HepG2 using 20451-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 20451-1-AP

    WB analysis of K-562 using 20451-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20451-1-AP

    IP experiment of HEK-293 using 20451-1-AP

    IP result of anti-SMN (IP:20451-1-AP, 4ug; Detection:20451-1-AP 1:300) with HEK-293 cells lysate 2000 ug.

  • IHC staining of human kidney using 20451-1-AP

    IHC staining of human kidney using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20451-1-AP

    IHC staining of human kidney using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human heart using 20451-1-AP

    IHC staining of human heart using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20451-1-AP (SMN antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 20451-1-AP

    IHC staining of human heart using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20451-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20451-1-AP

    IF Staining of HepG2 using 20451-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (20451-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HepG2 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20451-1-AP is SMN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMN fusion protein Ag14333 相同性解析による交差性が予測される生物種
Full Name survival of motor neuron 2, centromeric
Calculated molecular weight 282 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
RRIDAB_10665425
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Protocols

Product Specific Protocols
WB protocol for SMN antibody 20451-1-APDownload protocol
IHC protocol for SMN antibody 20451-1-APDownload protocol
IF protocol for SMN antibody 20451-1-APDownload protocol
IP protocol for SMN antibody 20451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols