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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC35A2 Polyclonal antibody

SLC35A2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13657-1-AP

Synonyms

SLC35A2, UDP Gal Tr, UDP galactose translocator, UDP galactose transporter, UGALT, UGAT, UGT, UGT1, UGT2, UGTL

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 13657-1-AP

    WB analysis of HEK-293 using 13657-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13657-1-AP (SLC35A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 13657-1-AP

    WB analysis of mouse brain using 13657-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13657-1-AP (SLC35A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13657-1-AP

    IHC staining of human liver cancer using 13657-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13657-1-AP

    IHC staining of human liver cancer using 13657-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13657-1-AP

    IHC staining of human colon using 13657-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 13657-1-AP

    IHC staining of human colon using 13657-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13657-1-AP targets SLC35A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC35A2 fusion protein Ag4524 相同性解析による交差性が予測される生物種
Full Name solute carrier family 35 (UDP-galactose transporter), member A2
Calculated molecular weight 396 aa, 41 kDa
Observed molecular weight 41 kDa, 32 kDa
GenBank accession numberBC035747
Gene symbol SLC35A2
Gene ID (NCBI) 7355
RRIDAB_2877967
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC35A2 antibody 13657-1-APDownload protocol
IHC protocol for SLC35A2 antibody 13657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Cell Oncol (Dordr)

Nucleotide sugar transporter SLC35A2 is involved in promoting hepatocellular carcinoma metastasis by regulating cellular glycosylation

Authors - Hongxia Cheng
  • KD Validated
View Article
IHC

Front Immunol

Comprehensive analysis of 33 human cancers reveals clinical implications and immunotherapeutic value of the solute carrier family 35 member A2

Authors - Shengshan Xu
View Article
humanIHC,WB

Aging (Albany NY)

Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer

Authors - Yue Wang
View Article
humanIHC,WB

FEBS J

SLC35A2 expression drives breast cancer progression via ERK pathway activation

Authors - Xiaochen Yang
View Article
humanWB

Gene

Solute carrier family 35 member A2 regulates mitophagy through the PI3K/AKT/mTOR axis, promoting the proliferation, migration, and invasion of osteosarcoma cells

Authors - Xiaohui Luo
View Article