Rat IL1-beta ELISA Kit0 Publications

カタログ番号: KE20005

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Rat IL1-beta ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
12.5 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Rat Serum 84% 77%-97%
Cell culture supernatants 107% 93%-118%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 76.4 3.0 4.0
2 20 243.0 10.2 4.2
3 20 849.2 30.1 4.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 50.1 4.2 8.3
2 24 190.3 7.3 3.8
3 24 684.7 17.4 2.5

製品概要
KE20005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes human IL1-beta. A polyclonal antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
IL-1, IL-1 beta, Il1b, interleukin 1 beta, Interleukin-1 beta
背景説明

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL1 gene family encodes three proteins: IL1 -alpha IL1- beta and their naturally occurring inhibitor IL1-RN. Interleukin 1 beta(IL1- beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL1-beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL1-beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.


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