Rat IL2 ELISA Kit0 Publications

カタログ番号: KE20002

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Rat IL2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
8.3 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 106% 93%-119%
Rat Serum 97% 83%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 38.4 2.4 6.2
2 20 219.4 9.8 4.5
3 20 1,013.1 62.1 6.1

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 37.0 2.3 6.2
2 24 225.5 17.2 7.6
3 24 996.8 84.6 8.5

製品概要
KE20002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2. The assay recognizes mouse IL2. A polyclonal antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Il2, Il-2, interleukin 2
背景説明

Interleukin-2 (IL2) is a protein initially identified as a T cell growth factor. IL2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that IL2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (IL2R). IL2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.


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